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KMID : 0880220140520070559
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Journal of Microbiology
2014 Volume.52 No. 7 p.559 ~ p.565
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Molecular characterization of atoxigenic Aspergillus flavus isolates collected in China
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Wei Dandan
Zhou Lu
Selvaraj Jonathan Nimal
Zhang Chushu
Xing Fuguo
Zhao Yueju
Wang Yan
Liu Yang
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Abstract
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Aspergillus flavus strains were isolated frompeanut fields of Liaoning, Shandong, Hubei and Guangdong Provinces in China, and identified through phenotypic and molecular approaches. Of the 323 A. flavus strains isolated, 76 strains did not produce aflatoxins detectable by UPLC. The incidence of atoxigenic A. flavus strains decreased with increase in temperature and increased with increase in latitude in different geographical locations. Amplification of all the aflatoxin genes in the aflatoxin gene cluster in the atoxigenic isolates showed that there were 25 deletion patterns (A?Y), with 22 deletion patterns identified for the first time. Most of the atoxigenic A. flavus isolates with gene deletions (97%) had deletions in at least one of the four genes (aflT, nor-1, aflR, and hypB), indicating that these four genes could be targeted for rapid identification of atoxigenic strains. The atoxigenic isolates with gene deletions, especially the isolates with large deletions, are potential candidates for aflatoxin control.
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KEYWORD
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aflatoxins, Aspergillus flavus, atoxigenic isolate, deletion pattern, gene cluster, biological control
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